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Clothes Minded Games: The Anesthesiology News Report

Headlines from MedPage Today® - Wed, 11/19/2014 - 3:33pm
(MedPage Today) -- A regular update on what's new in anesthesiology from our friends at Anesthesiology News.

Drug trial supports importance of low cholesterol to treat heart disease

news@nature - Wed, 11/19/2014 - 3:21pm

Results bolster the practice of using medicines to drive down cholesterol levels.

Nature News doi: 10.1038/nature.2014.16360

Hot Topics: Stem-Cell Tx for Multiple Myeloma

Headlines from MedPage Today® - Wed, 11/19/2014 - 3:07pm
(MedPage Today) -- Decision to defer stem-cell transplant after successful induction therapy remains controversial.

Philae: life and times of a comet lander

news@nature - Wed, 11/19/2014 - 2:21pm

Rosetta mission images reveal spacecraft activity before hibernation.

Nature News doi: 10.1038/nature.2014.16363

Pacemakers Get Hacked on TV, but Could It Happen in Real Life?

Headlines from MedPage Today® - Wed, 11/19/2014 - 2:20pm
(MedPage Today) -- Concern about the vulnerability of medical devices is growing.

Hypewatch: 100 Million Diabetic Americans in the Dark?

Headlines from MedPage Today® - Wed, 11/19/2014 - 1:52pm
(MedPage Today) -- News outlets incorrectly report the fraction of Americans with undiagnosed diabetes.

TNF-Inhibitors May Cut Risk of Congestive Heart Failure in RA

Headlines from MedPage Today® - Wed, 11/19/2014 - 1:00pm
(MedPage Today) -- Risk is lower than those patients taking nbDMARDs

Cyclophosphamide May Ease Idiopathic Inflammatory Myopathies

Headlines from MedPage Today® - Wed, 11/19/2014 - 12:21pm
(MedPage Today) -- Majority of patients treated with CYC experienced improved muscle strength and function.

Cellular 'computers' gain a hard drive

news@nature - Wed, 11/19/2014 - 12:21pm

DNA-based memory can record multiple inputs from engineered gene circuits.

Nature News doi: 10.1038/nature.2014.16343

Joint effort nabs next wave of US supercomputers

news@nature - Wed, 11/19/2014 - 12:21pm

National laboratories collaborate to purchase top-flight machines.

Nature 515 324 doi: 10.1038/nature.2014.16347

GastroBreak: Stool Bank, Difficult Diagnoses

Headlines from MedPage Today® - Wed, 11/19/2014 - 12:17pm
(MedPage Today) -- Both PCPs and specialists struggle to diagnose the etiology of constipation.

Hot Topics: COPD Tests and Treatment Planning

Headlines from MedPage Today® - Wed, 11/19/2014 - 11:15am
(MedPage Today) -- How do pulmonary function test results influence treatment in COPD?

UMCCTS Newsletter, November 2014

eScholarship@UMMS - Wed, 11/19/2014 - 11:06am

This is the November 2014 issue of the UMass Center for Clinical and Translational Science Newsletter containing news and events of interest.

ACR: Otezla Benefit Durable in PsA

Headlines from MedPage Today® - Wed, 11/19/2014 - 11:00am
(MedPage Today) -- Benefits persist through 2 years with apremilast in psoriatic arthritis.

AHA: PCSK9 Inhibitor Helps in Statin Intolerance (CME/CE)

Headlines from MedPage Today® - Wed, 11/19/2014 - 10:30am
(MedPage Today) -- But can novel agent alirocumab solve the side-effect dropout issue?

AHA: No Benefit to CABG, Mitral Valve Repair Combo (CME/CE)

Headlines from MedPage Today® - Wed, 11/19/2014 - 10:00am
(MedPage Today) -- Improvement in a marker of positive cardiac remodeling was not statistically different.

ASN Extras: Antibiotics Don't Block BK, Sickle Cell Ups CKD Risk (CME/CE)

Headlines from MedPage Today® - Wed, 11/19/2014 - 9:40am
(MedPage Today) -- Additional highlights from ASN's late-breaking presentations and journal publications.

Green List promotes best conservation areas

news@nature - Wed, 11/19/2014 - 9:21am

Project puts spotlight on protected reserves that boost biodiversity.

Nature 515 322 doi: 10.1038/nature.2014.16350

Phosphorylation of rhodopsin by protein kinase C in vitro

eScholarship@UMMS - Wed, 11/19/2014 - 9:18am

Calium/phospholipid-dependent protein kinase (protein kinase C) was purified from bovine retinae rod outer segments (ROS). In the presence of 0.1-2 microM calcium protein kinase C binds tightly to ROS and phosphorylates rhodopsin in the absence or presence of illumination. This property of protein kinase C contrasts with that of rhodopsin kinase, which in vitro phosphorylates only bleached rhodopsin. Peptide maps of rhodopsin phosphorylated by protein kinase C or rhodopsin kinase were compared using limited Staphylococcus aureus V8 protease digestion or complete tryptic digestion. Phosphorylation sites map to serine and threonine residues on the cytoplasmic carboxylterminal domain of rhodopsin for both kinases. The functional consequence of protein kinase C phosphorylation of rhodopsin was a reduced ability to stimulate the light-dependent rhodopsin activation of [35S]guanosine 5'-O-(thiotriphosphate) binding to transducin, the GTP-binding regulatory protein present in ROS. Properties of the calcium-stimulated interaction of protein kinase C with membranes and in vitro phosphorylation of intrinsic proteins are discussed based upon the findings.

Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography

eScholarship@UMMS - Wed, 11/19/2014 - 9:18am

The receptor for nerve growth factor (NGF) has been purified to near homogeneity from octylglucoside extracts of A875 melanoma cell membranes by the use of repetitive affinity chromatography on NGF-Sepharose. Elution of purified receptor (NGF receptor) was accomplished with 0.15 M NaCl, pH 11.0, containing phosphatidylcholine and octylglucoside. Chromatography on two columns of NGF-Sepharose yielded a 1500-fold purification of the receptor, as assessed by 125I-NGF binding, and permitted recovery of 9% of the total binding activity in the soluble extract. Scatchard analysis of equilibrium binding of 125I-NGF provided similar Kd values for NGF receptors in soluble extracts of A875 membranes (2.2 nM) and with purified NGF receptor (3.1 nM). Examination of NGF receptor after electrophoresis on sodium dodecyl sulfate-polyacrylamide gels revealed the presence of two major peptides, of Mr = 85,000 and Mr = 200,000. Affinity labeling experiments, done with 125I-NGF and A875 cells, soluble extracts of A875 cell membranes, and purified receptor, show that both of these components of the NGF receptor can be specifically cross-linked to 125I-NGF.

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